Exploring the induction of doubled haploids in cassava through gynogenesis

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The Use of Doubled-haploids in Cassava Breeding

Cassava breeding is difficult and, compared with other crops, inefficient. The problems in cassava breeding relate to the length of the breeding cycle, the large genetic load present in the crop, and the heterozygous nature of the parents and progenies evaluated. The production of doubled haploids, through tissue culture techniques, offers interesting advantages. By definition, any process that...

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Haploids and Doubled Haploids in Plant Breeding

Haploids are plants (sporophytes) that contain a gametic chromosome number (n). They can originate spontaneously in nature or as a result of various induction techniques. Spontaneous development of haploid plants has been known since 1922, when Blakeslee first described this phenomenon in Datura stramonium (Blakeslee et al., 1922); this was subsequently followed by similar reports in tobacco (N...

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Production and identification of doubled haploids in tropical maize.

The production of maize doubled haploid (DH) lines is a technique commonly used by private companies, but not by Brazilian public institutions. Research on this technique is essential to develop and improve the production of DH lines grown under tropical conditions. We assessed the ability of a gynogenetic haploid inducer system to induce haploids in a tropical environment, assessed the inducti...

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Thermal induction of diploid gynogenesis

Sporadic diploid gynogenetic fry resulted from fertilization with y-irradiated sperm. Their frequency increased appreciably when thermal shock treatments, beginning during the first hour of development (―0.4 OC lasting 6 hrs 45 min ; 26-30 °C lasting 10 min), were used. When the eggs were fertilized with normal sperm, a good triploidization rate was induced by means of heat shocks (27-30...

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Doubled haploids for studying the inheritance of quantitative characters.

By using a doubled-haploid population derived from F2 plants, additive and additive X additive genetic variances, as well as the number of segregating genes, can be estimated. An F2-derived doubled-haploid population may contain almost 50% more of the best recombinant than an F1-derived population. However, the best recombinant occurs in the same frequency in the two populations when there is n...

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ژورنال

عنوان ژورنال: African Journal of Agricultural Research

سال: 2019

ISSN: 1991-637X

DOI: 10.5897/ajar2018.13779